Journal: Bio-protocol
Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)
doi: 10.21769/BioProtoc.5304
Figure Lengend Snippet: (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy medium (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.
Techniques: Derivative Assay, Control, Staining, Marker, Expressing