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mem non-essential amino acids solution (mem-neaa), 100  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mem non-essential amino acids solution (mem-neaa), 100
    Mem Non Essential Amino Acids Solution (Mem Neaa), 100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non-essential+amino+acids+(mem+neaa+(100)/mem+non+essential+amino+acids/pm39915620-419-57-65
    Average 90 stars, based on 1 article reviews
    mem non-essential amino acids solution (mem-neaa), 100 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)
    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Article Title: Effects of whey protein treatment in an in vitro intestinal cell model following oxidative stress or inflammatory challenge
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: The protective roles of eugenol on type 1 diabetes mellitus through NRF2-mediated oxidative stress pathway
    Article Snippet: The mouse pancreatic β cell line MIN6 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and was cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, United States) supplemented with 1% penicillin/streptomycin (Gibco, CA, USA), 10% fetal bovine serum (Gibco, CA, USA), and 1% MEM NON-ESSENTIAL AMINO ACIDS SOLUTION (100×; NEAA, Gibco, USA) at a temperature of 37°C in a CO 2 incubator with a concentration of 5%.

    Article Title: Puromycin Proximity Ligation Assay (Puro-PLA) to Assess Local Translation in Axons From Human Neurons
    Article Snippet: MEM non-essential amino acids solution (100×) (MEM-NEAA) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 9.

    Article Title: Size-Dependent Cytotoxicity and Multi-Omic Changes Induced by Amorphous Silicon Nanoparticles in HepG2 Cells
    Article Snippet: HepG2 cells were grown in Minimum Essential Medium (MEM, Gibco, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, Thermo Scientific, New York, NY, USA), 1% MEM Non-Essential Amino Acids Solution (100×) (NEAA, Gibco, Thermo Scientific, New York, NY, USA), and 2% GlutaMAX-1 (Gibco, Thermo Scientific, New York, NY, USA) and maintained at 37 °C in a humidified atmosphere containing 5% CO 2 .

    Article Title: Soluble CD95L is a prognostic marker in Central Nervous System Post-Transplant Lymphoproliferative Disorders.
    Article Snippet: PBMCs were thawed in RPMI+ medium (100 UI/mL penicillin / 100 μg/mL streptomycin / 0.25 μg/mL Amphotericin B, 1 mM sodium pyruvate, 0.1 mM MEM NEAA, 2mM L-glutamine; Thermo Fisher Scientific) supplemented with 20% FBS (Biowest), then washed twice with RPMI+ medium 10% FBS and suspended in proper media for each specific test.

    Article Title: Effects of triclosan on cytokine production by human immune cells in vitro.
    Article Snippet: After, the cells were resuspended in Hams F12 media (Nutrient Mixture, prod# 21765–029, Gibco, Thermo Fischer Scientific, Paisley, UK), supplemented with 1 % FBS, 1 % L-glutamine (prod# 25030-081, Gibco, Thermo Fischer Scientific, Paisley, UK), 1 % penicillin–streptomycin (prod# 15140, Gibco, Thermo Fischer Scientific, New York, US) and 1 % non-essential amino acids (MEM NEAA (100×), ref#11140–035, Gibco, Thermo Fischer Scientific, Paisley, UK).

    Control:

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)
    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Article Title: Effects of whey protein treatment in an in vitro intestinal cell model following oxidative stress or inflammatory challenge
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: The protective roles of eugenol on type 1 diabetes mellitus through NRF2-mediated oxidative stress pathway
    Article Snippet: The mouse pancreatic β cell line MIN6 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and was cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, United States) supplemented with 1% penicillin/streptomycin (Gibco, CA, USA), 10% fetal bovine serum (Gibco, CA, USA), and 1% MEM NON-ESSENTIAL AMINO ACIDS SOLUTION (100×; NEAA, Gibco, USA) at a temperature of 37°C in a CO 2 incubator with a concentration of 5%.

    Article Title: Puromycin Proximity Ligation Assay (Puro-PLA) to Assess Local Translation in Axons From Human Neurons
    Article Snippet: MEM non-essential amino acids solution (100×) (MEM-NEAA) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 9.

    Article Title: Size-Dependent Cytotoxicity and Multi-Omic Changes Induced by Amorphous Silicon Nanoparticles in HepG2 Cells
    Article Snippet: HepG2 cells were grown in Minimum Essential Medium (MEM, Gibco, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, Thermo Scientific, New York, NY, USA), 1% MEM Non-Essential Amino Acids Solution (100×) (NEAA, Gibco, Thermo Scientific, New York, NY, USA), and 2% GlutaMAX-1 (Gibco, Thermo Scientific, New York, NY, USA) and maintained at 37 °C in a humidified atmosphere containing 5% CO 2 .

    Article Title: Soluble CD95L is a prognostic marker in Central Nervous System Post-Transplant Lymphoproliferative Disorders.
    Article Snippet: PBMCs were thawed in RPMI+ medium (100 UI/mL penicillin / 100 μg/mL streptomycin / 0.25 μg/mL Amphotericin B, 1 mM sodium pyruvate, 0.1 mM MEM NEAA, 2mM L-glutamine; Thermo Fisher Scientific) supplemented with 20% FBS (Biowest), then washed twice with RPMI+ medium 10% FBS and suspended in proper media for each specific test.

    Article Title: Effects of triclosan on cytokine production by human immune cells in vitro.
    Article Snippet: After, the cells were resuspended in Hams F12 media (Nutrient Mixture, prod# 21765–029, Gibco, Thermo Fischer Scientific, Paisley, UK), supplemented with 1 % FBS, 1 % L-glutamine (prod# 25030-081, Gibco, Thermo Fischer Scientific, Paisley, UK), 1 % penicillin–streptomycin (prod# 15140, Gibco, Thermo Fischer Scientific, New York, US) and 1 % non-essential amino acids (MEM NEAA (100×), ref#11140–035, Gibco, Thermo Fischer Scientific, Paisley, UK).

    Staining:

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)
    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Article Title: Effects of whey protein treatment in an in vitro intestinal cell model following oxidative stress or inflammatory challenge
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: The protective roles of eugenol on type 1 diabetes mellitus through NRF2-mediated oxidative stress pathway
    Article Snippet: The mouse pancreatic β cell line MIN6 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and was cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, United States) supplemented with 1% penicillin/streptomycin (Gibco, CA, USA), 10% fetal bovine serum (Gibco, CA, USA), and 1% MEM NON-ESSENTIAL AMINO ACIDS SOLUTION (100×; NEAA, Gibco, USA) at a temperature of 37°C in a CO 2 incubator with a concentration of 5%.

    Article Title: Puromycin Proximity Ligation Assay (Puro-PLA) to Assess Local Translation in Axons From Human Neurons
    Article Snippet: MEM non-essential amino acids solution (100×) (MEM-NEAA) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 9.

    Article Title: Size-Dependent Cytotoxicity and Multi-Omic Changes Induced by Amorphous Silicon Nanoparticles in HepG2 Cells
    Article Snippet: HepG2 cells were grown in Minimum Essential Medium (MEM, Gibco, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, Thermo Scientific, New York, NY, USA), 1% MEM Non-Essential Amino Acids Solution (100×) (NEAA, Gibco, Thermo Scientific, New York, NY, USA), and 2% GlutaMAX-1 (Gibco, Thermo Scientific, New York, NY, USA) and maintained at 37 °C in a humidified atmosphere containing 5% CO 2 .

    Article Title: Soluble CD95L is a prognostic marker in Central Nervous System Post-Transplant Lymphoproliferative Disorders.
    Article Snippet: PBMCs were thawed in RPMI+ medium (100 UI/mL penicillin / 100 μg/mL streptomycin / 0.25 μg/mL Amphotericin B, 1 mM sodium pyruvate, 0.1 mM MEM NEAA, 2mM L-glutamine; Thermo Fisher Scientific) supplemented with 20% FBS (Biowest), then washed twice with RPMI+ medium 10% FBS and suspended in proper media for each specific test.

    Article Title: Effects of triclosan on cytokine production by human immune cells in vitro.
    Article Snippet: After, the cells were resuspended in Hams F12 media (Nutrient Mixture, prod# 21765–029, Gibco, Thermo Fischer Scientific, Paisley, UK), supplemented with 1 % FBS, 1 % L-glutamine (prod# 25030-081, Gibco, Thermo Fischer Scientific, Paisley, UK), 1 % penicillin–streptomycin (prod# 15140, Gibco, Thermo Fischer Scientific, New York, US) and 1 % non-essential amino acids (MEM NEAA (100×), ref#11140–035, Gibco, Thermo Fischer Scientific, Paisley, UK).

    Marker:

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)
    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Article Title: Effects of whey protein treatment in an in vitro intestinal cell model following oxidative stress or inflammatory challenge
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: The protective roles of eugenol on type 1 diabetes mellitus through NRF2-mediated oxidative stress pathway
    Article Snippet: The mouse pancreatic β cell line MIN6 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and was cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, United States) supplemented with 1% penicillin/streptomycin (Gibco, CA, USA), 10% fetal bovine serum (Gibco, CA, USA), and 1% MEM NON-ESSENTIAL AMINO ACIDS SOLUTION (100×; NEAA, Gibco, USA) at a temperature of 37°C in a CO 2 incubator with a concentration of 5%.

    Article Title: Puromycin Proximity Ligation Assay (Puro-PLA) to Assess Local Translation in Axons From Human Neurons
    Article Snippet: MEM non-essential amino acids solution (100×) (MEM-NEAA) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 9.

    Article Title: Size-Dependent Cytotoxicity and Multi-Omic Changes Induced by Amorphous Silicon Nanoparticles in HepG2 Cells
    Article Snippet: HepG2 cells were grown in Minimum Essential Medium (MEM, Gibco, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, Thermo Scientific, New York, NY, USA), 1% MEM Non-Essential Amino Acids Solution (100×) (NEAA, Gibco, Thermo Scientific, New York, NY, USA), and 2% GlutaMAX-1 (Gibco, Thermo Scientific, New York, NY, USA) and maintained at 37 °C in a humidified atmosphere containing 5% CO 2 .

    Article Title: Soluble CD95L is a prognostic marker in Central Nervous System Post-Transplant Lymphoproliferative Disorders.
    Article Snippet: PBMCs were thawed in RPMI+ medium (100 UI/mL penicillin / 100 μg/mL streptomycin / 0.25 μg/mL Amphotericin B, 1 mM sodium pyruvate, 0.1 mM MEM NEAA, 2mM L-glutamine; Thermo Fisher Scientific) supplemented with 20% FBS (Biowest), then washed twice with RPMI+ medium 10% FBS and suspended in proper media for each specific test.

    Article Title: Effects of triclosan on cytokine production by human immune cells in vitro.
    Article Snippet: After, the cells were resuspended in Hams F12 media (Nutrient Mixture, prod# 21765–029, Gibco, Thermo Fischer Scientific, Paisley, UK), supplemented with 1 % FBS, 1 % L-glutamine (prod# 25030-081, Gibco, Thermo Fischer Scientific, Paisley, UK), 1 % penicillin–streptomycin (prod# 15140, Gibco, Thermo Fischer Scientific, New York, US) and 1 % non-essential amino acids (MEM NEAA (100×), ref#11140–035, Gibco, Thermo Fischer Scientific, Paisley, UK).

    Expressing:

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)
    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Article Title: Effects of whey protein treatment in an in vitro intestinal cell model following oxidative stress or inflammatory challenge
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: The protective roles of eugenol on type 1 diabetes mellitus through NRF2-mediated oxidative stress pathway
    Article Snippet: The mouse pancreatic β cell line MIN6 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and was cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, United States) supplemented with 1% penicillin/streptomycin (Gibco, CA, USA), 10% fetal bovine serum (Gibco, CA, USA), and 1% MEM NON-ESSENTIAL AMINO ACIDS SOLUTION (100×; NEAA, Gibco, USA) at a temperature of 37°C in a CO 2 incubator with a concentration of 5%.

    Article Title: Puromycin Proximity Ligation Assay (Puro-PLA) to Assess Local Translation in Axons From Human Neurons
    Article Snippet: MEM non-essential amino acids solution (100×) (MEM-NEAA) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 9.

    Article Title: Size-Dependent Cytotoxicity and Multi-Omic Changes Induced by Amorphous Silicon Nanoparticles in HepG2 Cells
    Article Snippet: HepG2 cells were grown in Minimum Essential Medium (MEM, Gibco, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, Thermo Scientific, New York, NY, USA), 1% MEM Non-Essential Amino Acids Solution (100×) (NEAA, Gibco, Thermo Scientific, New York, NY, USA), and 2% GlutaMAX-1 (Gibco, Thermo Scientific, New York, NY, USA) and maintained at 37 °C in a humidified atmosphere containing 5% CO 2 .

    Article Title: Soluble CD95L is a prognostic marker in Central Nervous System Post-Transplant Lymphoproliferative Disorders.
    Article Snippet: PBMCs were thawed in RPMI+ medium (100 UI/mL penicillin / 100 μg/mL streptomycin / 0.25 μg/mL Amphotericin B, 1 mM sodium pyruvate, 0.1 mM MEM NEAA, 2mM L-glutamine; Thermo Fisher Scientific) supplemented with 20% FBS (Biowest), then washed twice with RPMI+ medium 10% FBS and suspended in proper media for each specific test.

    Article Title: Effects of triclosan on cytokine production by human immune cells in vitro.
    Article Snippet: After, the cells were resuspended in Hams F12 media (Nutrient Mixture, prod# 21765–029, Gibco, Thermo Fischer Scientific, Paisley, UK), supplemented with 1 % FBS, 1 % L-glutamine (prod# 25030-081, Gibco, Thermo Fischer Scientific, Paisley, UK), 1 % penicillin–streptomycin (prod# 15140, Gibco, Thermo Fischer Scientific, New York, US) and 1 % non-essential amino acids (MEM NEAA (100×), ref#11140–035, Gibco, Thermo Fischer Scientific, Paisley, UK).

    Cell Culture:

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)
    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Article Title: Effects of whey protein treatment in an in vitro intestinal cell model following oxidative stress or inflammatory challenge
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: The protective roles of eugenol on type 1 diabetes mellitus through NRF2-mediated oxidative stress pathway
    Article Snippet: The mouse pancreatic β cell line MIN6 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and was cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, United States) supplemented with 1% penicillin/streptomycin (Gibco, CA, USA), 10% fetal bovine serum (Gibco, CA, USA), and 1% MEM NON-ESSENTIAL AMINO ACIDS SOLUTION (100×; NEAA, Gibco, USA) at a temperature of 37°C in a CO 2 incubator with a concentration of 5%.

    Article Title: Puromycin Proximity Ligation Assay (Puro-PLA) to Assess Local Translation in Axons From Human Neurons
    Article Snippet: MEM non-essential amino acids solution (100×) (MEM-NEAA) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 9.

    Article Title: Size-Dependent Cytotoxicity and Multi-Omic Changes Induced by Amorphous Silicon Nanoparticles in HepG2 Cells
    Article Snippet: HepG2 cells were grown in Minimum Essential Medium (MEM, Gibco, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, Thermo Scientific, New York, NY, USA), 1% MEM Non-Essential Amino Acids Solution (100×) (NEAA, Gibco, Thermo Scientific, New York, NY, USA), and 2% GlutaMAX-1 (Gibco, Thermo Scientific, New York, NY, USA) and maintained at 37 °C in a humidified atmosphere containing 5% CO 2 .

    Article Title: Soluble CD95L is a prognostic marker in Central Nervous System Post-Transplant Lymphoproliferative Disorders.
    Article Snippet: PBMCs were thawed in RPMI+ medium (100 UI/mL penicillin / 100 μg/mL streptomycin / 0.25 μg/mL Amphotericin B, 1 mM sodium pyruvate, 0.1 mM MEM NEAA, 2mM L-glutamine; Thermo Fisher Scientific) supplemented with 20% FBS (Biowest), then washed twice with RPMI+ medium 10% FBS and suspended in proper media for each specific test.

    Article Title: Effects of triclosan on cytokine production by human immune cells in vitro.
    Article Snippet: After, the cells were resuspended in Hams F12 media (Nutrient Mixture, prod# 21765–029, Gibco, Thermo Fischer Scientific, Paisley, UK), supplemented with 1 % FBS, 1 % L-glutamine (prod# 25030-081, Gibco, Thermo Fischer Scientific, Paisley, UK), 1 % penicillin–streptomycin (prod# 15140, Gibco, Thermo Fischer Scientific, New York, US) and 1 % non-essential amino acids (MEM NEAA (100×), ref#11140–035, Gibco, Thermo Fischer Scientific, Paisley, UK).

    Modification:

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)
    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Article Title: Effects of whey protein treatment in an in vitro intestinal cell model following oxidative stress or inflammatory challenge
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: The protective roles of eugenol on type 1 diabetes mellitus through NRF2-mediated oxidative stress pathway
    Article Snippet: The mouse pancreatic β cell line MIN6 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and was cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, United States) supplemented with 1% penicillin/streptomycin (Gibco, CA, USA), 10% fetal bovine serum (Gibco, CA, USA), and 1% MEM NON-ESSENTIAL AMINO ACIDS SOLUTION (100×; NEAA, Gibco, USA) at a temperature of 37°C in a CO 2 incubator with a concentration of 5%.

    Article Title: Puromycin Proximity Ligation Assay (Puro-PLA) to Assess Local Translation in Axons From Human Neurons
    Article Snippet: MEM non-essential amino acids solution (100×) (MEM-NEAA) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 9.

    Article Title: Size-Dependent Cytotoxicity and Multi-Omic Changes Induced by Amorphous Silicon Nanoparticles in HepG2 Cells
    Article Snippet: HepG2 cells were grown in Minimum Essential Medium (MEM, Gibco, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, Thermo Scientific, New York, NY, USA), 1% MEM Non-Essential Amino Acids Solution (100×) (NEAA, Gibco, Thermo Scientific, New York, NY, USA), and 2% GlutaMAX-1 (Gibco, Thermo Scientific, New York, NY, USA) and maintained at 37 °C in a humidified atmosphere containing 5% CO 2 .

    Article Title: Soluble CD95L is a prognostic marker in Central Nervous System Post-Transplant Lymphoproliferative Disorders.
    Article Snippet: PBMCs were thawed in RPMI+ medium (100 UI/mL penicillin / 100 μg/mL streptomycin / 0.25 μg/mL Amphotericin B, 1 mM sodium pyruvate, 0.1 mM MEM NEAA, 2mM L-glutamine; Thermo Fisher Scientific) supplemented with 20% FBS (Biowest), then washed twice with RPMI+ medium 10% FBS and suspended in proper media for each specific test.

    Article Title: Effects of triclosan on cytokine production by human immune cells in vitro.
    Article Snippet: After, the cells were resuspended in Hams F12 media (Nutrient Mixture, prod# 21765–029, Gibco, Thermo Fischer Scientific, Paisley, UK), supplemented with 1 % FBS, 1 % L-glutamine (prod# 25030-081, Gibco, Thermo Fischer Scientific, Paisley, UK), 1 % penicillin–streptomycin (prod# 15140, Gibco, Thermo Fischer Scientific, New York, US) and 1 % non-essential amino acids (MEM NEAA (100×), ref#11140–035, Gibco, Thermo Fischer Scientific, Paisley, UK).

    Concentration Assay:

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)
    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Article Title: Effects of whey protein treatment in an in vitro intestinal cell model following oxidative stress or inflammatory challenge
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: The protective roles of eugenol on type 1 diabetes mellitus through NRF2-mediated oxidative stress pathway
    Article Snippet: The mouse pancreatic β cell line MIN6 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and was cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, United States) supplemented with 1% penicillin/streptomycin (Gibco, CA, USA), 10% fetal bovine serum (Gibco, CA, USA), and 1% MEM NON-ESSENTIAL AMINO ACIDS SOLUTION (100×; NEAA, Gibco, USA) at a temperature of 37°C in a CO 2 incubator with a concentration of 5%.

    Article Title: Puromycin Proximity Ligation Assay (Puro-PLA) to Assess Local Translation in Axons From Human Neurons
    Article Snippet: MEM non-essential amino acids solution (100×) (MEM-NEAA) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 9.

    Article Title: Size-Dependent Cytotoxicity and Multi-Omic Changes Induced by Amorphous Silicon Nanoparticles in HepG2 Cells
    Article Snippet: HepG2 cells were grown in Minimum Essential Medium (MEM, Gibco, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, Thermo Scientific, New York, NY, USA), 1% MEM Non-Essential Amino Acids Solution (100×) (NEAA, Gibco, Thermo Scientific, New York, NY, USA), and 2% GlutaMAX-1 (Gibco, Thermo Scientific, New York, NY, USA) and maintained at 37 °C in a humidified atmosphere containing 5% CO 2 .

    Article Title: Soluble CD95L is a prognostic marker in Central Nervous System Post-Transplant Lymphoproliferative Disorders.
    Article Snippet: PBMCs were thawed in RPMI+ medium (100 UI/mL penicillin / 100 μg/mL streptomycin / 0.25 μg/mL Amphotericin B, 1 mM sodium pyruvate, 0.1 mM MEM NEAA, 2mM L-glutamine; Thermo Fisher Scientific) supplemented with 20% FBS (Biowest), then washed twice with RPMI+ medium 10% FBS and suspended in proper media for each specific test.

    Article Title: Effects of triclosan on cytokine production by human immune cells in vitro.
    Article Snippet: After, the cells were resuspended in Hams F12 media (Nutrient Mixture, prod# 21765–029, Gibco, Thermo Fischer Scientific, Paisley, UK), supplemented with 1 % FBS, 1 % L-glutamine (prod# 25030-081, Gibco, Thermo Fischer Scientific, Paisley, UK), 1 % penicillin–streptomycin (prod# 15140, Gibco, Thermo Fischer Scientific, New York, US) and 1 % non-essential amino acids (MEM NEAA (100×), ref#11140–035, Gibco, Thermo Fischer Scientific, Paisley, UK).



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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
    Mem Non Essential Amino Acids Solution (Neaa) 100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non-essential+amino+acids+(mem+neaa+(100)/mem+non+essential+amino+acids+solution/pmc12067298-103-0-7
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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    Image Search Results


    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy medium (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.

    Journal: Bio-protocol

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)

    doi: 10.21769/BioProtoc.5304

    Figure Lengend Snippet: (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy medium (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.

    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Techniques: Derivative Assay, Control, Staining, Marker, Expressing